Figure 3

GF triggers mitochondria dysfunction and caspases activation. (A) Mito-Tracker Red staining shows the mitochondrial morphology and potential changes upon GF treatment. The LNCaP cells were treated with 10 μM GF for 12 h and 24 h. After GF treatment in serum-free medium, changes in mitochondria were scanned by laser confocal microscopy. Scale bar, 20 μm. (B) Western blot showed that the activation of caspase 3, 7, 9 and PARP protein in LNCaP cells treated with 10 μM for different time. GF triggers mitochondia-mediated apoptosis in LNCaP cells. (C) Western blot showed that the activation of caspase 3, 7, 9 and PARP protein in PC3 cells treated with 10 μM GF for different time.