Electron microscopic demonstration of alginate beads with HCT116 spheroids. The HCT116 cells are embedded (A) and divided (B-C) in alginate beads (*) after 3-7days. They move apart forming more and more aggregates (arrow) and a capsule. The round to oval HCT116 cells cultured in alginate for 10-14 days (D-E), showing cell aggregates within a channel (arrows), proliferating cells emigrating from the beads. Numerous cells were apoptotic and fragmented (arrowheads). x4.500.