Figure 4

Tenovin-6 induces apoptosis in ALL cells. A, REH and NALM-6 cells were treated with increasing concentrations of Tenovin-6 for 24 or 48 hours. Apoptosis was determined by flow cytometry after Annexin V-FITC/propidium iodide staining. Representative histograms (left) and results for 3 independent experiments (right) are shown. B, Dead cells (including apoptotic and necrotic cells) in primary cells from children with ALL were examined by flow cytometry after treatment with Tenovin-6 for 48 hours. Histograms from 2 representative patients (left) and statistical analysis in 3 patients (right) are shown. * P < 0.05, ** P <0.01, one-way ANOVA, post hoc comparisons, Tukey’s test; error bars represent 95% CIs. C, REH and NALM-6 cells were treated with the indicated concentrations of Tenovin-6 for 24 or 48 hours. Time- and dose-dependent cleavage of PARP and levels of pro-caspase 3, Mcl-1, and XIAP were detected by Western blotting.