Figure 3
Tenovin-6 inhibits the growth of ALL cells. A, REH and NALM-6 ALL cells were exposed to Tenovin-6 for 72 hours. Cell viability (percentage relative to control) was determined by MTS assay. Tenovin-6 dose–response curves are shown. B & C, Mononuclear cells from peripheral blood of 46 children with primary (“Initial”) or relapsed ALL and from bone marrow of 5 healthy individuals (normal bone marrow; NBM) were exposed to increasing concentrations of Tenovin-6 and then subjected to MTS assay. Representative dose–response curves (B) and IC50 values of Tenovin-6 for all patients and healthy individuals (C) are shown. D, Tenovin-6 inhibited the clonogenicity of ALL cells. REH and NALM-6 cells were seeded in soft agar with the indicated concentrations of Tenovin-6 for 14 days, and then colony-forming units were counted. * P < 0.05, ** P <0.01, one-way ANOVA, post hoc comparisons, Tukey’s test; error bars represent 95% CIs. E, Colony-forming capacity of primary ALL bone marrow cells from 4 children with ALL and 3 normal bone marrow cells were evaluated by using methylcellulose medium with the indicated concentration of Tenovin-6. A representative curve is shown. F, Cell cycle distributions in REH and NALM-6 cells after exposure to increasing concentrations of Tenovin-6. * P < 0.05 compared with control.