Table 2 Antibodies used in the present study

Primary antibodies

Host

Manufacturer*

Cat. no.

Dilution IHC/ IF

Dilution WB/DB

β-Actin

Rabbit polycolnal IgG

Cell Signaling

4967

-

1:1000

Laminin

Rabbit polycolnal IgG

Dako

Z0097

1:200

-

Laminin α1 (H-300)

Rabbit polycolnal IgG

Santa Cruz

sc-5582

-

1:200

Laminin β1 (H-300)

Rabbit polycolnal IgG

Santa Cruz

sc-5583

1:50

1:200

Laminin 5

Rabbit polycolnal IgG

Abcam

Ab14509

1:100

-

Laminin-5 (P3E4)

Mouse monoclonal IgG1

Santa Cruz

sc-13587

1:50

1:200

Fibronectin

Rabbit polycolnal IgG

Dako

A0245

1:100

-

Fibronectin (IST-9)

Mouse monoclonal IgG1

Santa Cruz

sc-59826

1:40

1:500

Smoth Muscle Actin colone 1A4

Mouse monoclonal IgG1

Dako

M0851

1:200

-

Thrombospondin 1 (A6.1)

Mouse monoclonal IgG1

Santa Cruz

sc-59887

-

1:250

Perlecan

Mouse monoclonal IgG1

Zymed

13-4400

-

1:500

Collagen I

Rabbit polycolnal IgG

Abcam

ab34710

-

1:1000

Collagen III

Rabbit polycolnal IgG

Abcam

Ab7778

-

1:1000

Anti-Human Collagen IV

Mouse monoclonal IgG1

DakoCytomation

M 0785

-

1:200

Anti-Human MMP-7

Mouse monoclonal IgG_1K

Chemicon

MAB3315

-

1:500

TIMP1 (D10E6)

Rabbit monoclonal IgG

Cell Signaling

8946

-

1:1000

TIMP3 (D74B10)

Rabbit monoclonal IgG

Cell Signaling

5673

-

1:1000

Integrin α4

Rabbit polycolnal IgG

Cell Signaling

4600

-

1:1000

Integrin α5

Rabbit polycolnal IgG

Cell Signaling

4705

-

1:1000

Integrin α6

Rabbit polycolnal IgG

Cell Signaling

3750

-

1:750

Integrin αV

Rabbit polycolnal IgG

Cell Signaling

4711

-

1:1000

Integrin β1

Rabbit polycolnal IgG

Cell Signaling

4706

-

1:1000

Integrin β3

Rabbit polycolnal IgG

Cell Signaling

4702

-

1:100

Integrin β4

Rabbit polycolnal IgG

Cell Signaling

4707

-

1:1000

CD44

Mouse monoclonal IgG2a

Antibodies online

ABIN96695

1:100

1:500

CD151 (PETA-3)

Mouse monoclonal IgG2b

Novocastra™ Leica Biosystems

NCL-CD151

1:50

-

Secondary antibodies

Host

Manufacturer*

Cat. no.

Dilution IHC/IF

Dilution WB/DB

Anti-mouse Ig/HRP

Goat polyclonal

DakoCytomation

P0447

-

1:2000

Anti-rabbit Ig/HRP

Goat polyclonal

DakoCytomation

P0448

-

1:2000

Alexa Fluor® 647 anti-mouse IgG (H + L)

Donkey polyclonal

Invitrogen

A31571

1:200

-

Alexa Fluor® 488 anti-mouse IgG (H + L)

Donkey polyclonal

Invitrogen

A21202

1:200

-

Alexa Fluor® 647 anti-rabbit IgG (H + L)

Goat polyclonal

Invitrogen

A21244

1:200

-

Alexa Fluor® 568 anti-rabbit IgG (H + L)

Goat polyclonal

Invitrogene

A11011

1:200

-