Figure 4

Secreted matrix protein profile of normal and tumor associated fibroblasts growing alone or in co-culture with CSCC7 cells. Dot blots were prepared from the conditioned medium of NF, TF and CSCC7 in monoculture (A), from direct co-culture of NF + CSCC7 and TF + CSCC7 (B), from the indirect co-culture fibroblast side of TF/CSCC7 and NF/CSCC7 (C), and the indirect co-culture cancer cell side of TF/CSCC7 and NF/CSCC7 (D). Blots indicate reaction with antibodies against the proteins. The difference in secretory activity between normal and tumorous fibroblasts was assessed. Stars indicate significance: *p < 0.05, **p < 0.001.