Figure 4

Involvement of PI3K pathway, but not Akt, in cisplatin-induced splicing. A-F: MCF7 cells were treated with TGX221 (5 μM; A-B) or IC87114 (10 μM; C-D) three hours prior to treatment with cisplatin (50 μM, 24 hours). E-H: MCF7 cells were treated with triciribine (20 μM) or MK2206 (2 μM) three hours before cisplatin treatment (50 μM; 24 hours). Alternative splicing of exon 6 of HNRNPDL (B,D,F,H) and exon 3 of AMZ2 (A,C,E,G) was evaluated by RT-PCR. RT-PCR products were fractionated by gel electrophoresis.