Figure 4

Effect of IMP3 is independent of IGF-2 and NGFβ. Cell lysates were subjected to immunoprecipitation using anti-human IMP3 and rabbit IgG. RNA extracted from the precipitates were analyzed using qRT-PCR. (A) Higher amplifications of IGF-2 mRNA and NGFβ mRNA were detected in IMP3 pull-down fraction relative to IgG. (B) Treatment with IMP3 siRNA did not alter the mRNA levels of IGF-2 while it increased NGFβ mRNA relative to scrambled siRNA-treated counterparts. qRT-PCR was used to measure mRNA levels following siRNA transfection. (C) Reduction in IMP3 did not alter levels of IGF-2 and NGFβ protein in Hs766T. Intracellular protein levels were measured using ELISAs. (D) Knocking down IMP3 did not alter the levels of phosphorylated and total ERK as well as phosphorylated and total AKT.