Effect of IMP3 knockdown on motility, invasion, and matrix adhesion of Hs766T. (A) The motility of Hs766T in Boyden chambers was significantly decreased following siRNA-mediated inhibition of IMP3. Inset Representative images (10X) of motile Hs766T 24 h after seeding. (B) The invasive potential of Hs766T was evaluated using Matrigel-coated Boyden chambers. IMP3 depletion resulted in a significant decrease in the invasive potential of Hs766T. Inset Representative images (10X) of invasive Hs766T 24 h after seeding. (C) Adhesion to collagen I (Coll I), collagen II (Coll II), collagen IV (coll IV), fibronectin (FN), tenascin (TN), laminin (LN), tenascin (TN), and vitronectin (VN) was quantified spectrophotometrically. Absorbance at 550 nm is proportional to the number of adherent cells. *P < 0.05, **P < 0.01 relative to scrambled siRNA-transfected controls.