Figure 2

Cyclin D1 expression in HCMLs alters various molecular functions. A. The whole-genome expression patterns for 8226 D1-GFP vs. 8226 GFP (left) and LP1 D1-GFP vs. LP1 GFP (right) are shown as heat maps. Each column represents a sample; each row represents a marker (gene transcript). The log2 relative gene expression scale is shown at the bottom left. The differentially expressed genes (Fc threshold ≥ 1.5, q = 0.01) were classified as cyclin D1-activated genes (in red) or cyclin D1-inhibited genes (in green). B. Real-time quantitative RT-PCR for the validation of microarray data. The sequences of the primers used for RT-PCR are indicated in the Additional file 1. For each sample, the mean Ct value for the internal standard (RPLP0) was subtracted from the mean Ct value for each gene, to obtain the ΔΔCt. The formula N = 2^-ΔΔCt was used to calculate the Fc in expression. The experiments were carried out three times; mean ± SD values are indicated in the table. For each gene, the Fc calculated from microarray data is indicated in the column on the right. C. Genes for which expression levels were altered (Fc > 2) by cyclin D1 expression were ordered by function-related groups, for both cell lines. The number of genes with altered expression levels corresponding to each cell function is indicated on the graph.