Figure 5

Expression of sumoylation-resistant Kif18A induces aberrant BubR1 localization in anaphase cells. (A) HeLa cells were transfected with a plasmid expressing GFP-WT for 48 h, after which cells were fixed and stained with the antibody against BubR1 (red). DNA was stained with DAPI (blue). Representative metaphase cell images are shown in the upper panel. In the lower panel, HeLa cells transiently expressing GFP-WT or GFP-5R were fixed and stained with the BubR1 antibody (red). DNA was stained with DAPI (blue). Representative anaphase cell images are shown. (B) Percentage of anaphase cells with aberrant (signals in anaphase) BubR1 localization were recorded and plotted. Data were summarized from three independent experiments (WT, n = 85; 5R, n = 94; vector, n = 76). Asterisk indicates statistically significant difference between mutant 5R and WT (or vector).