Figure 2

The same variant inNBNas detected by Sanger sequencing (A) and NGS (B). Note that the frequency of alleles at variable positions, read depth in independent forward and reverse reads and quality scores can be reviewed here. The heterozygous base change indicated by the arrow in panel A is the same base change selected in the NGS results in panel B. NBN, NBN-associated cancer risk ; NGS, next generation sequencing.