CA7 expression is frequently downregulated in CRC. (A) The expression levels of CA7 mRNA in 59 paired human primary CRC tissues (T) and adjacent normal tissues (N) were evaluated by real-time qPCR methods. (T vs N, p < 0.001) (B) Protein levels of CA7 in an independent set of 25 paired CRC specimens and adjacent normal tissues were determined by Western blot assay. β-actin was used as a loading control. The relative protein expression of CA7 was quantified and normalized to β-actin. Each N was arbitrarily designated 1.0. (T: Tumor; N: adjacent normal tissues, T vs N, p < 0.001) (C) Representative immunohistochemical expression patterns of CA7 in cancerous and adjacent normal mucosa specimens were shown. (Magnification, left panel, ×100; right panel, ×400) (D) Percentage of patients with different staining intensity of CA7 in the tumor or adjacent normal tissues in the training cohort (p < 0.001).