Figure 5

Four HCC cell lines (HUH7, HCCLM3, SMMC7721, LO2) were treated with 5-aza-dCyd, a methyltransferase inhibitor and the miRNA expression levels were assayed using TaqMan miRNA PCR. The expression of mir-372 was restored with 5-aza-dCyd treatment in HUH7 and HCCLM3 cell lines (*P < 0.05,**P < 0.01) and the mir-372 DNA methylation level was inhibited. Treatment with a histone deacetylase inhibitor, TSA, had no influence on the expression of mir-372 in all four cell lines.