Hsp90b1 is upregulated in CLL patients with the rs2307842 polymorphism andIGHV-unmutated status, as assessed by qRT-PCR and western blot analysis. Box plots show the relative upregulation of HSP90B1 mRNA in CLL patients with (A) rs2307842 (VAR) and (B) IGHV unmutated genes (UM) compared with wild-type CLL patients (WT) and the mutated cases (MUT), respectively. The thick line inside the box plot indicates the median expression levels and the box shows the 25th and 75th percentiles, while the whiskers show the maximum and minimum values. Outliers are represented by open circles. Statistical significance was determined by the Mann–Whitney U test (P < 0.05). (C) Representative lysates of purified B lymphocytes from CLL patients were prepared and Hsp90b1 protein levels were analyzed by western blot. B-actin served as loading control. Representative blots from three CLL patients are shown: #1 patient with IGHV unmutated genes (UM CLL), #2 wild-type for rs2307842 and with IGHV mutated genes (WT&MUT CLL) and #3 patient with rs2307842 (VAR CLL).