Figure 6

Barasertib induces apoptotic cell death of fulvestrant resistant cell lines. A-B. Parental (T47D) and fulvestrant resistant (182^R-1 and 182^R-2) cells were treated with barasertib (50 nM), cisplatin (20 μM) or DMSO (control) for 96 hours, stained with SYTOX green and analyzed by fluorescence microscopy and flow cytometry. Percentages of dead (SYTOX green positive) cells are indicated in bold C. Western blots showing total and cleaved form of PARP in lysates from T47D, 182^R-1 and 182^R-2 cells treated with barasertib (50 nM) or DMSO (control) for 4–96 hours. Heat shock protein 70 (Hsp70) was used as loading control. Two individual experiments were performed and data from one representative experiment are shown.