Figure 4
Barasertib prevents chromosome alignment in fulvestrant resistant cell lines. Fluorescence microscopy of Hoechst stained (AandD) parental, (BandE) 182R-1 and (CandF) 182R-2 T47D cells treated for 42 hours with DMSO (control; A-C) or barasertib (50 nM; D-F). Inserts show higher-magnification images of dividing cells.The experiment was repeated twice and representative images are shown.