Non-supervised hierarchical clustering obtained with public datasets. A. The combined expression of the profile genes did not correctly distinguish between pCR and non-pCR tumors treated with a docetaxel-based chemotherapy. B. The same observation was made with a trastuzumab monotherapy regimen. C. In contrast, pCR were distinguished from non-pCR tumors (accuracy of 85%) when tumors were treated with a first-line neoadjuvant trastuzumab + docetaxel-based regimen. The vertical red dashed line represents the separation between the 2 response subgroups. D. The use of first-line neoadjuvant chemotherapy before treatment with trastuzumab + docetaxel altered the prediction capacity of our profile. Green and red colors represent underexpression or overexpression centered on median array values, respectively.