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Table 2 Percentage of thyroid carcinoma cells determined by FACS analysis in each cell cycle phase following 24 h of treatment with sorafenib or vehicle

From: Sorafenib inhibits intracellular signaling pathways and induces cell cycle arrest and cell death in thyroid carcinoma cells irrespective of histological origin or BRAF mutational status

Cell line Type Status %SubG1 %G1 %G2/M %S
BHT101 Papillary Unstimulated 4.5 ± 0.6 58.3 ± 3.9 17.7 ± 0.9 24.0 ± 1.3
  Sorafenib 24 h 60.2 ± 6.4* 72.1 ± 5.8* 13.4 ± 0.7* 14.5 ± 0.9*
B-CPAP Papillary Unstimulated 6.8 ± 2.3 57.1 ± 2.7 14.0 ± 1.1 28.9 ± 1.4
  Sorafenib 24 h 62.3 ± 7.3* 69.8 ± 4.6* 23.1 ± 1.2* 7.1 ± 0.4*
TPC1 Papillary Unstimulated 3.1 ± 0.4 48.1 ± 3.5 25.3 ± 1.6 26.6 ± 1.3
  Sorafenib 24 h 72.4 ± 5.9* 57.6 ± 4.2* 27.6 ± 3.5 14.8 ± 0.8*
FTC133 Follicular Unstimulated 1.5 ± 0.3 57.0 ± 3.5 13.7 ± 0.8 29.3 ± 1.5
  Sorafenib 24 h 22.1 ± 3.4* 46.9 ± 4.4* 8.7 ± 0.5* 44.4 ± 2.9*
FTC236 Follicular Unstimulated 1.2 ± 0.2 64.1 ± 5.0 12.1 ± 0.9 23.8 ± 1.1
  Sorafenib 24 h 35.2 ± 3.7* 36.0 ± 3.1* 15.6 ± 1.0* 48.4 ± 3.7*
FTC238 Follicular Unstimulated 0.7 ± 0.1 46.1 ± 2.9 9.3 ± 0.6 44.6 ± 5.8
  Sorafenib 24 h 43.0 ± 5.0* 24.2 ± 1.4* 11.0 ± 1.7 64.6 ± 6.6*
ML1 Follicular Unstimulated 1.0 ± 0.1 56.5 ± 3.2 18.1 ± 1.2 25.4 ± 1.3
  Sorafenib 24 h 40.4 ± 3.2* 59.9 ± 2.7 25.5 ± 1.8* 14.4 ± 0.8*
TT2609 Follicular Unstimulated 1.6 ± 0.2 57.6 ± 4.6 11.5 ± 1.3 30.9 ± 1.8
  Sorafenib 24 h 21.5 ± 3.0* 54.7 ± 6.0 13.1 ± 1.6 32.2 ± 3.1
SW1736 Anaplastic Unstimulated 2.2 ± 0.3 46.0 ± 2.9 10.1 ± 1.1 43.9 ± 2.7
  Sorafenib 24 h 53.6 ± 3.9* 68.1 ± 5.5* 9.7 ± 0.7 22.2 ± 1.2*
C643 Anaplastic Unstimulated 1.0 ± 0.1 50.5 ± 3.7 15.1 ± 0.8 34.4 ± 2.8
  Sorafenib 24 h 43.8 ± 4.0* 38.6 ± 2.6* 11.5 ± 0.5* 49.9 ± 3.5*
HTh7 Anaplastic Unstimulated 5.9 ± 0.8 52.2 ± 3.5 14.5 ± 0.8 33.3 ± 1.8
  Sorafenib 24 h 25.7 ± 3.4* 76.9 ± 6.4* 1.2 ± 0.1* 21.9 ± 0.9*
HTh83 Anaplastic Unstimulated 5.6 ± 0.3 36.5 ± 2.6 24.3 ± 1.7 39.2 ± 2.0
  Sorafenib 24 h 74.7 ± 5.7* 35.0 ± 5.2 34.5 ± 1.9* 30.5 ± 1.5*
  1. Values for subG1 peaks represent the percentage of all cells measured, while values for G1-, G2/M- and S-phase are depicted for the remaining living cells. Values are given as mean values ± standard deviation of 6-fold determinations. *indicates significant changes (p<0.05, Student’s t-test).