Figure 3

Integrin expression levels across disease progression. A) cDNA from several different prostate-derived cell lines was analyzed using semi-quantitative RT-PCR. GAPDH amplification was used as a loading control. B) Gene expression omnibus dataset GDS3289 investigating prostate cancer progression in LCM-captured clinical samples was interrogated for EPHB4 and ITGB8 expression using the Oncomine clinical database (www.oncomine.org). Both genes are significantly elevated in prostatic intraepithelial neoplasia (PIN) and EPHB4 is significantly upregulated in carcinoma samples compared to benign. * p < 0.001, mets = metastatic disease.