Effects of IGFBP7-containing conditioned media on EOC cell lines. A, Immunoblot analysis monitoring IGFBP7 protein levels in the CM from EOC cell lines. Recombinant IGFBP7 protein was used as a positive control and two different exposures are shown (10 and 60 seconds). B, Spheroid formation assay of OV90 neor and TOV112D cell lines cultured in IGFBP7-containing CM (TOV21G and TOV81D) or control CM (TOV112D). Images are shown at × 20 magnification. C, Wound healing assay with OV90 neor and TOV112D cultured in IGFBP7-containing CM (TOV21G and TOV81D) or control CM (TOV112D) observed over a period of 6 days. Images are shown at × 10 magnification.