Figure 2From: Cyproheptadine, an antihistaminic drug, inhibits proliferation of hepatocellular carcinoma cells by blocking cell cycle progression through the activation of P38 MAP kinaseEffects of cyproheptadine on the cell cycle in HCC cells. HepG2 (A) and Huh-7 (B) cells in 6-well plates were cultured for 24 h, starved in serum-free medium for 24 h, and then treated with cyproheptadine at 30 or 40 μM (HepG2) or at 25 or 35 μM (Huh-7) for 48 h. Treated cells were stained with propidium iodide and analyzed by flow cytometry. Data are presented as mean ± SD (n = 4). Significant differences from the no-treatment control, determined by one-way ANOVA and Dunnett’s comparison test, are indicated by asterisks: *p < 0.05; ***p < 0.001. No difference was observed between the no-treatment control and the DMSO-only control in all test groups, indicating the absence of confounding effects from the DMSO solvent.Back to article page