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Figure 4 | BMC Cancer

Figure 4

From: Astrocyte elevated gene-1(AEG-1) induces epithelial-mesenchymal transition in lung cancer through activating Wnt/β-catenin signaling

Figure 4

AEG-1 promoted Wnt-mediated EMT. Knockdown of AEG-1 activated GSK-3β, and inhibited β-catenin activity and EMT in NCI-H226 cells. Cells were co-transfected with AEG-1-siRNA and TOP or FOP. Then, cells were treated with Wnt-CM. (A) The expression of the indicated proteins was analyzed by Western blot in NCI-H226 (control siRNA) and AEG-1-siRNA with or without Wnt-3a-CM, respectively. (B) Relative luciferase expression of β-catenin was measured as described above. (C) and (D) In contrast, restoring AEG-1 expression in Slu-01 cells (AEG-1-negative) promoted Wnt-induced EMT.

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BMC Cancer

ISSN: 1471-2407

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