Figure 3

AEG-1 was associated with CKIδand modulated the GSK-3β/β-catenin signaling pathway. (A) Slu-01 cells stably overexpressing AEG-1 were established. GSK-3β was immunoprecipitated from cell lysates, and its expression was confirmed by immunoblotting with the indicated antibodies. (B) CKIδ was critical for AEG-1-mediated regulation of GSK-3β/β-catenin signaling and EMT. AEG-1 complexes were associated with both GSK-3β and CKIδ. (C) CKIδplayed a role in AEG-mediated regulation of EMT and Ser-9 phosphorylation of GSK-3β. Slu-01 cells transfected with pcDNA3.1-AEG-1 were co-transfected with CKIδ-specific siRNA. Cell lysates were then subjected to Western blotting.