Figure 4
IFN-β is responsible for the positive effect of IFN-positive CAFs on MCF-7 proliferation. Three IFN-positive CAFs, T38, T44, T65, were co-cultured as previously described. In this experiment, two new conditions where added: once triplicate for each day was reserved for the addition of 20 μg of an IgG1 isotype control antibody (second darkest shade) and another for 20 μg of neutralizing IFN-β antibody (darkest shade). A Bonferonni multiple comparison test was carried out after a one-way ANOVA was performed on the four conditions at 120 hours. The asterisks represent the significant difference between the co-culture with the IgG isotype control and the co-culture with the neutralizing IFN-β antibody (*, p < 0.05; ***, p < 0.001).