Figure 3
KMS12-PE myeloma cells reduce SA-βGalA and modify cell cycle characteristics of MM-BMMSCs. Asterisks indicate indicate p-values with * <0.05, ** < 0.01, *** < 0.001 and **** < 0.0001. All data were analyzed using Wilcoxon signed-rank test and paired t-test (ELISA analysis). (A) KMS12-PE myeloma cells reduce SA-βGalA in MM-BMMSCs upon co-cultivation (n = 20) and cultivation in transwells (n = 6). (i) The MFI in MM-BMMSCs was significantly reduced in both culture systems. No changes were observed for co-cultured HD-BMMSCs (n = 3) and HS-5 cells (n = 3) indicating specificity of the measured effect for MM-BMMSCs. (ii) Representative histogram of a co-cultured MM-BMMSC and transwell-cultured MM-BMMSC population compared to the same MM-BMMSC population cultured alone. (B) Cell interaction with KMS12-PE myeloma cells induced increased cyclin E1 and p21 expression in MM-BMMSCs compared to MM-BMMSCs cultured alone (co-culture n = 25, transwell culture n = 10). (C) Protein expression analysis of co-cultured MM-BMMSCs (n = 3) compared to mono-cultured MM-BMMSCs. Cyclin E1 was increased whereas cyclin D1 and p21 were reduced in co-cultured cells compared to mono-cultures. No change was seen for p16. (D) Cell interaction with KMS12-PE myeloma cells induced an increase of cells in G1/G0 phase and reduced the amount of cells in S phase in co-cultured and transwell cultured MM-BMMSCs (n = 8) compared to the same MM-BMMSCs cultured alone.