Figure 2

Activin inhibits breast cancer cell proliferation. MDA-MB −231 (A + C) and MCF7 (B + D) cells were treated with increasing doses of activin in a timecourse experiment (A + B), or with recombinant activin (6000 pg/ml) +/− follistatin (64,000 pg/ml) for 72 hours (C + D). 20 μl of MTS solution was added 4 hours prior to the final time points to evaluate absorbance of treated cells relative to control untreated cells. Data represents mean + SEM of 8 replicates and 5 repeats. Wilcoxon Signed-Rank test for significance, *p value <0.05, NS not significant.