Figure 8

Metformin reduces tumor weight and cancer cell proliferation. (A) 6606PDA cells were injected on day 0 into the pancreas of C57BL/6J mice. Between day 8 and day 29 PBS (Sham) or metformin was ip injected daily and tissue samples were analyzed on day 29. (B) Quantification of the tumor weight in the indicated mouse cohorts. (C) Quantification of BrdU⁺ nuclei within the carcinoma reveals that metformin treatment reduces cell proliferation. Box plots indicate the median, the 25^th and 75^th percentiles in the form of a box, and the 10^th and 90^th percentiles as whiskers. The number of animals evaluated was n = 8 (Sham) and n = 7 (metformin), Significant differences between the cohorts are indicated, *P = 0.004 (B), *P = 0.029 (C).