Table 1 List of primers used for analysis
Gene | Forward primer | Reverse primer |
|---|---|---|
FN | 5′-TGCCGCAACTACTGTGAT-3′ | 5′GAATCCTGGGCTGGAGTA--3′ |
G-CSF | 5′-CAGATCACCCAGAATCCAT-3′ | 5′-CTCTCGTCCTGACCATAGTG-3′ |
Jak3 | 5′-TGGCCACTGAGGACTTCTCT-3′ | 5′-GGATGGCACTGGTCAAATCT-3′ |
IL6 | 5′-ACAAGAAAGACAAAGCCAGA-3′ | 5′-TAGCCACTCCTTCTGTGACT-3′ |
GATA3 | 5′-CTGGAGGAGGAACGCTAATG-3′ | 5′-GTTGAAGGAGCTGCTCTTGG-3′ |
IL10 | 5′-TCTCCCCTGTGAAAATAAGA-3′ | 5′-TCCAGCAGACTCAATACACA-3′ |
Tyk2 | 5′-ATCCGTTTGTACAGGCCAAG-3′ | 5′-GCTGTGTGATGGGGAACTTT-3′ |
CD40 | 5′-GGCTTCGGGTTAAGAAGGAG-3′ | 5′-GCAGGGATGACAGACGGTAT-3′ |
CTLA4 | 5′-GGATCCTTGTCGCAGTTAGC-3′ | 5′-AAACGGCCTTTCAGTTGATG-3′ |
TGFβ | 5′-TGCGCTTGCAGAGATTAAAA-3′ | 5′-CGTCAAAAGACAGCCACTCA-3′ |
GAPDH | 5′-TCATCCCTGCATCCACTG-3′ | 5′-TAGGAACACGGAAGGCCA-3′ |