Figure 1

S100A4 activates T-cell differentiation pathwaysin vitro.(A) Relative expression of IL6, IL10, Jak3, Tyk2, CD40, CTLA4, GATA3 and TGF-β as determined by qRT-PCR of RNA isolated from T-cells treated with the S100A4 protein. *, P < 0.05; **, P < 0.01. (B) Phosphorylation of Jak3 in T-cells treated either with S100A4 protein, mutant S100A4 protein, S100A4 protein mixed with 6B12, or 6B12 alone. T-cells were stimulated for 5 and 10 min. Western blot analysis with anti-phospho-Jak3 and anti-Jak3. (C) Phosphorylation of Stat3 in T-cells treated with S100A4 protein, mutant S100A4 protein, S100A4 protein mixed with 6B12, or 6B12 alone. T-cells were stimulated for 5 and 10 min. Western blot analysis with anti-phospho-Stat3 and anti-Stat3 antibodies.