Western blotting analysis of EphA5 protein expression in clinical prostate specimens. A, EphA5 protein (114 kDa band) levels in representative resected fresh prostate cancer tissues and matched non-tumor tissues were analyzed by western blotting and normalized to β-actin expression (43 kDa band). Expression was further normalized to the expression level observed in the first noncancerous specimen. B, EphA5 protein levels (114 kDa band) in representative fresh prostate cancer tissues (collected via biopsy) and benign prostate hyperplasias were analyzed by western blotting and normalized to β-actin (43 kDa band). Protein levels were further normalized to the expression level observed in the first benign prostate hyperplasia specimen. Representative results from triplicate experiments are shown as mean ± SD (n = 3). *P < 0.05, vs. the respective noncancerous tissue and BPH. N = non-tumor tissue; T = tumor tissue; BPH = benign prostate hyperplasia.