qRT-PCR and Western blot analysis of EphA5 expression in prostate cell lines. A, EphA5 mRNA expression was analyzed by qRT-PCR in 7 prostate cell lines. GAPDH was amplified as an internal control. B, Western blot analysis of EphA5 protein (114 kDa band) expression in 7 prostate cell lines. β-Actin (43 kDa band) was used as a control for equal loading of cell lysates. Representative results of triplicate experiments are shown as mean ± SD (n = 3). *P < 0.05, vs. the RWPE-1 cell line; **P < 0.05, vs. the LNCaP cell line, ***P < 0.05, vs. the PC-3 cell line.