Figure 6

TOX3 regulates estrogen-responsive genes in a ligand-independent manner. A. Overlap of genes upregulated by TOX3 (yellow) in MCF-7 cells as shown here, and those previously reported to be bound by ER and/or be regulated by estrogen [31] (blue). B. TFF1 mRNA expression in transiently transfected MCF-7 cells under estrogen depleted conditions. Data are expressed as mean ± SD of three independent experiments. C. TFF1 gene expression in stably transfected MCF-7 cell lines. D. TFF1 pre-mRNA expression in indicated cell lines treated with estrogen (E2) or vehicle (EtOH). E. eRNA expression in stably transfected MCF-7 cells. Data are expressed as mean ± SD of three independent experiments. F. Estrogen-induced TFF1 mRNA expression following siRNA-mediated TOX3 knockdown as indicated. Data are expressed as mean ± SD of six independent experiments. G. TFF1 expression following siRNA mediated ER knockdown in MCF-7 stable cells lines as indicated in presence of estrogen (E2) or vehicle (EtOH). Data are expressed as mean ± SD of four independent experiments. H. TFF1 expression after ER and/or TOX3 transfection of MDA-MB-231 cells and with indicated additions. Data are expressed as mean ± SD of four independent experiments.