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Table 3 Transcript expression by qRT-PCR in relation to protein expression and gene amplification using IHC and CISH/FISH methodologies, respectively.

From: Quantitative real-time RT-PCR and chromogenic in situ hybridization: precise methods to detect HER-2 status in breast carcinoma

   qRT-PCR
  n (%) HER-2ratio (range) * P Ratio
≤ 2.00
n (%)
Ratio
>2.00
n (%)
IHC      
0 or 1+ 50 (66.7) 0.93 (0.05–12.24) <0.0001a 40 (80.0) 10 (20.0)
2+ 12 (17.3) 2.46 (1.15–7.61)   5 (41.7) 7 (58.3)
3+ 13 (16.0) 7.55 (1.07–20.43)   1 (7.7) 12 (92.3)
Total 75     
CISH      
No amplification 24 (64.9) 0.75 (0.05–1.95) <0.0001b 24 (100) 0 (0.0)
High amplification 13 (35.1) 4.69 (1.07–20.43)   2 (15.4) 11 (84.6)
Total 37     
FISH      
No amplification 0 (0.0) 0 (0.0) ND 0 (0.0) 0 (0.0)
Amplification 8 (100) 7.93 (2.64–20.43)   0 (0.0) 8 (100)
Total 8     
  1. * Mean HER-2/reference gene ratios; ND: not determined.
  2. a Kruskal-Wallis test.
  3. b Mann-Whitney test.