Table 3 Transcript expression by qRT-PCR in relation to protein expression and gene amplification using IHC and CISH/FISH methodologies, respectively.
qRT-PCR | |||||
|---|---|---|---|---|---|
n (%) | HER-2ratio (range) * | P | Ratio ≤ 2.00 n (%) | Ratio >2.00 n (%) | |
IHC | |||||
0 or 1+ | 50 (66.7) | 0.93 (0.05–12.24) | <0.0001a | 40 (80.0) | 10 (20.0) |
2+ | 12 (17.3) | 2.46 (1.15–7.61) | 5 (41.7) | 7 (58.3) | |
3+ | 13 (16.0) | 7.55 (1.07–20.43) | 1 (7.7) | 12 (92.3) | |
Total | 75 | ||||
CISH | |||||
No amplification | 24 (64.9) | 0.75 (0.05–1.95) | <0.0001b | 24 (100) | 0 (0.0) |
High amplification | 13 (35.1) | 4.69 (1.07–20.43) | 2 (15.4) | 11 (84.6) | |
Total | 37 | ||||
FISH | |||||
No amplification | 0 (0.0) | 0 (0.0) | ND | 0 (0.0) | 0 (0.0) |
Amplification | 8 (100) | 7.93 (2.64–20.43) | 0 (0.0) | 8 (100) | |
Total | 8 | ||||