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Table 1 Comparison of clinicopathological features with HER-2 status using CISH, qRT-PCR (qPCR), and IHC.

From: Quantitative real-time RT-PCR and chromogenic in situ hybridization: precise methods to detect HER-2 status in breast carcinoma

Variables (n)a CISH P * qPCR P * IHC P *
  ≤ 5 >10   ≤ 2.00 >2.00   0/1+ 2/3+  
Age (years)          
≤ 50 (28) 9 5 0.9541 15 13 0.2569 17 11 0.3651
>50 (48) 15 8   32 16   34 14  
Lymph node status          
<4 (57) 21 9 0.1756 38 19 0.0915 40 17 0.4723
≥ 4 (32) 3 4   8 10   11 7  
ND (1) 0 0   0 1   0 1  
Tumor Size (cm)          
0 – 2 (19) 5 5 0.1883 10 9 0.2953 11 8 0.2745
>2 (56) 19 7   37 19   40 16  
ND (1) 0 1   0 1   0 1  
Clinical Stage          
I/IIA/IIB (65) 21 9 0.1756 41 24 0.5901 44 21 0.7912
IIIA/IIIB (11) 3 4   6 5   7 4  
Histologic grade          
I/II (36) 10 6 1.000 22 14 0.9237 23 13 0.6767
III (35) 10 6   21 14   24 11  
ND (5) 4 1   4 1   4 1  
Ki-67 status          
Low (≤ 25%) (32) 11 6 0.5133 19 13 0.5354 21 11 0.7209
High (> 25%) (31) 8 7   16 15   19 12  
Not reactive (13) 5 0   12 1   11 2  
Familial History of Cancer          
Yes (15) 4 3 0.6346 7 8 0.1769 10 5 0.9678
No (61) 20 10   40 21   41 20  
  1. a Number of the cases evaluated by qRT-PCR and IHC, while 37 cases were evaluated using CISH.
  2. * Chi-Square test.
  3. ND: not determined.