Mitotic and apoptotic indexes and tumor weight. A) Mitosis. B) Apoptosis. Slides were prepared from criopreserved tumors. Respectively, were analyzed slides from tumors for each condition derived from 3 mice at 7 days, 8 mice at 14 days, 6 mice at 21 days and 9 mice at 28 days. Slides were stained with Giemsa and with Hoechst stain. The results were similar for either stain and here are presented the data from Giemsa stained slides. For each slide at least 1000 cells were screened in randomly chosen fields, for a total of cells varying from 3000 to 9000 per point in each condition. Bars indicate S.D. C) Weight in mg of dissected tumors. 3 tumors for each condition were analyzed at 7, 24, 31, 38 and 48 days, and from 6 to 9 tumors for all other time points in each condition. Bars indicate S.D and the asterisks show the significance by one-way ANOVA. White columns are PBS-mock treatment controls and black columns are PICP-treated tumors.