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Table 2 Phenotypic and genetic features of GFAP+NNP and glioblastoma cell cultures

From: Arrested neural and advanced mesenchymal differentiation of glioblastoma cells-comparative study with neural progenitors

Feature GBM1 GBM2 GBM3 GBM4 GBM5 GBM6 GBM7 GBM8 NHA
% of multilineage phenotype cells in original culture 95% 70% 63% 45% 30% 10% 15% 45% 100%
Other than multilineage phenotypes observed at the beginning of in vitro culture glial, neuronal-intermediate, mesenchymal glial, mesenchymal glial, mesenchymal glial, mesenchymal glial, neuronal-intermediate, mesenchymal glial, mesenchymal other, not defined glial, neuronal-intermediate, neuronal, mesenchymal 0%
Clonable - + - + - + - - -
% of CD133+ cells 0% 3% 0% 2% 2% 1% 2% 3% 0%
Aggregate-forming ability + + + + - - - + +
Aggregates stability + + + + nt nt nt + -
Mesenchymal differentiation * (+) 10 (+) 15 (+) 10 (+) 15 (+) 10 (+) 10 - (+) 10 (+)5
Molecular background EGFR+, P53wt EGFR-, P53mut EGFR+, P53wt EGFR-, P53wt EGFR+, P53wt EGFR-, P53mut EGFR-, P53 wt EGFR-, P53mut EGFR-, P53wt
  1. The phenotypes: multilineage phenotype, in brief CD44+/MAP2/GFAP+ (in detail Beta III tubulin+/Nestin+/Vimentin+/SOX2+); glial, GFAP+/CD44+/MAP2-; neuronal, MAP2+/GFAP-/CD44-; neuronal-intermediate, GFAP+/MAP2+/CD44-; mesenchymal, in brief CD44+/GFAP-/MAP2- (in detail CD44+/Vimentin+/Fibronectin+/GFAP-/MAP2-/Beta III-tubulin-/Nestin-/SOX2-).
  2. EGFR+, amplification of EGFR gene; EGFR-, no amplification of EGFR gene; P53wt, wild type; P53mut, mutation in P53; nt, not tested.
  3. GBM, glioblastoma
  4. *Number of passages required to present only mesenchymal (non-neural) cells.