Table 2 Phenotypic and genetic features of GFAP+NNP and glioblastoma cell cultures
Feature | GBM1 | GBM2 | GBM3 | GBM4 | GBM5 | GBM6 | GBM7 | GBM8 | NHA |
|---|---|---|---|---|---|---|---|---|---|
% of multilineage phenotype cells in original culture | 95% | 70% | 63% | 45% | 30% | 10% | 15% | 45% | 100% |
Other than multilineage phenotypes observed at the beginning of in vitro culture | glial, neuronal-intermediate, mesenchymal | glial, mesenchymal | glial, mesenchymal | glial, mesenchymal | glial, neuronal-intermediate, mesenchymal | glial, mesenchymal | other, not defined | glial, neuronal-intermediate, neuronal, mesenchymal | 0% |
Clonable | - | + | - | + | - | + | - | - | - |
% of CD133+ cells | 0% | 3% | 0% | 2% | 2% | 1% | 2% | 3% | 0% |
Aggregate-forming ability | + | + | + | + | - | - | - | + | + |
Aggregates stability | + | + | + | + | nt | nt | nt | + | - |
Mesenchymal differentiation * | (+) 10 | (+) 15 | (+) 10 | (+) 15 | (+) 10 | (+) 10 | - | (+) 10 | (+)5 |
Molecular background | EGFR+, P53wt | EGFR-, P53mut | EGFR+, P53wt | EGFR-, P53wt | EGFR+, P53wt | EGFR-, P53mut | EGFR-, P53 wt | EGFR-, P53mut | EGFR-, P53wt |