Figure 2From: Arrested neural and advanced mesenchymal differentiation of glioblastoma cells-comparative study with neural progenitors Molecular characterization of glioblastomas. a, Western blot analysis to show MAP2, Nestin and GFAP expression in cultured GBM1, GBM2, GBM3, GBM4 (lanes 1â4) cells and control brain lane 6; lane 5, negative control blood cells. b, Multiplex analysis allowing to detect EGFR amplification. Lanes 1, 2, 3 samples showing amplification of EGFR; lane 4, negative control; lane 5, positive control. c, Sequencing of P53 normal sample and sample presenting mutation of P53 (GBM6). d, LOH analysis. Lane a, blood sample; lane b, tumor sample frozen tissue contaminated with cells presenting ROH (ROH, retention of heterozygosity); lane c, lack of cells with ROH, DNA isolated from aggregate.Back to article page