CtBP1 re-expressing melanoma cell clones. An expression plasmid for CtBP1 was introduced into Mel Im cells by stable transfection. (A) Expression of CtBP1 protein was analyzed by western blotting. Expression of full length CtBP1 was observed in the CtBP1-transfected cell clone CtBP1#3, CtBP1#6 and CtBP1#8 but not in the wild-type Mel Im cell line or in the mock transfected cell clones Mock 1 and Mock 2. (B) Influence of CtBP1 expression on MIA mRNA expression was determined by quantitative RT-PCR. The three CtBP1 re-expressing clones (CtBP1#3, #6 and #8) showed strong reduction in MIA expression compared with mock transfected clones. (*: p < 0.05 compared to Mock 1).