HCCR-1 is positively well correlated with breast cancer prognostic parameters. Correlation of HCCR-1 expression levels with known breast cancer prognostic parameters. Immunohistochemical staining for ER, PR, HER2, p53 and HCCR-1 in fresh frozen tissues from human breast cancers. All the breast cancer tissues were derived from pathologically-proven invasive ductal carcinomas. Positive immunostaining for ER, PR, and p53 in the nuclei of carcinoma cells and for HCCR-1 in the cytoplasm of carcinoma cells (top panel). The HER2 immunohistochemical staining showing complete strong membrane staining (3+) in the carcinoma cells (top panel). Negative immunostaining for ER, PR, p53, HER2 and HCCR-1 in breast carcinoma (bottom panel). Original magnification, × 100.