Figure 5
From: Secretion of MCP-1 and other paracrine factors in a novel tumor-bone coculture model
Synergistic regulation of MCP-1 in cocultures. NCTC 2472 fibrosarcoma (Cells) or mouse osteosarcoma (K7M2) cells were cultured alone, neonatal femurs were cultured alone (BN) or these components were cultured together (Co-Cult) and media was collected at indicated times. Across four independent experiments, media was sampled from 8–9 wells for each time point, culture condition and media. Mean × 103 pg/ml of MCP-1 ± SE is shown for day 1 (white bar) and day 2 (black) of culture. A: A greater-than-additive increase in MCP-1 concentration in femur-fibrosarcoma cocultures is observed as compared to cultures of femur or cells alone, regardless of media conditions. Simple sum of [MCP-1]cells + [MCP-1]bn is indicated on or above the day 1 Co-Cult bar (dashed line) and on the day 2 Co-Cult bar (white line). Bracket indicates the difference between this sum and the actual [MCP-1] observed in cocultures which is statistically significant at P < 0.001(*). B: Cocultures conducted with the mouse osteosarcoma K7M2 cells, known to secrete MCP-1 and interact within the bone microenvironment, showed no synergistic regulation of MCP-1 regardless of culture conditions. Simple sum of [MCP-1] cells + [MCP-1]BN is indicated above the day 1 and day 2 Co-Cult bars (dashed line).