SMARCB1 mutation screening by HRM in tumors 3180 and 3161. Figure 1 shows HRM difference plots for; (a) exon 5 in tumor 3180, (b) exon 2 in tumor 3180 and (c) exon 6 in tumor 3161. The melting curves associated with the identified sequence variations (shown in red) were clearly distinguishable from the control DNA samples (green lines). HRM data for exon 5 in tumor 3161 is shown in Figure 2a. The melting curves shown represent the average melting data of grouped duplicate samples. (d) sequence traces for each mutation detected. The mutation position is indicated by *.