Figure 2

RT-PCR analysis of the expression of 6 markers in Detroit 562 and FaDu cells. Total RNA from the Detroit 562 and FaDu cell lines was reverse transcribed then used in individual reactions to amplify products corresponding to each marker as indicated. cDNA from the breast cancer cell line MDA-MB-468 was used as a positive control for EphB4, EGFR, EpCam and Elf3. No RT = negative control for the reverse transcription reaction to which no enzyme was added, No RNA = negative control for the reverse transcription reaction to which no RNA was added, PCR -ve = PCR reagent only negative control. Marker = pUC19/HpaII. The size of the amplified product is shown in base pairs (bp). A genomic band is seen above the indicated RT-PCR band for ELF3 and CEA.