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Table 1 Forward and reverse primers for the genes analyzed
| Gene | Forward primer | Reverse primer |
|---|---|---|
| HIF-1α | 5'CTGCTGTCTTACTGGTCCTT3' | 5'GTCGCTTCTCCA ATTCTTAC3' |
| COX-2 | 5'CCATTCAGTTCCCACCATCT3' | 5'TCACTGCTGTTGGGTCTCTG3' |
| MMP7 | 5'AGATGTGGAGTGCCAGATGT3' | 5'TAGACTGCTACCATCCGTCC3' |
| MMP9 | 5'GGCGCTCATGTACCCTATGT3' | 5'CCTGTGTACACCCACACCTG3' |
| VEGF | 5'ATGAACTTTCTGCTCTCTGG3' | 5'TCATCTCTCCTATGTGCTGGC3' |
| PDGFRA | 5'GGGGAAACGATTGTGGTCACC3' | 5'CCCGCACCTCTACAACAAAAT3' |
| MYC | 5'AAAGGCCCCCAAGGTAGTTA3' | 5'TTTCCGCAACAAGTCCTCTT3' |
| HPRT | 5'CCTGGCGTCGTGATTAGTG3' | 5'CAGAGGGCTACAATGTGATGG3' |
| TBP | 5'ACCACTCCACTGTATCCCTCC3' | 5'CTGTTCTTCACTCTTGGCTCCT3' |
