Figure 1

Effect of IFNγ on indoleamine 2,3-dioxygenase (IDO) expression in the nasopharyngeal carcinoma cell line, CNE2. (A) and (B) IFNγ-induced IDO protein expression in CNE2 cells assessed by immunohistochemistry. CNE2 cells were treated with (A) or without (B) 50 U/ml IFNγ for 24 h, and stained with anti-IDO antibody. The images were captured at ×100 magnification, and ×400 magnification for inserts. (C) and (D) IFNγ induced IDO protein expression in CNE2 cells in a dose- and time-dependent manner. CNE2 cells were cultured in the presence of indicated concentrations of IFNγ for 24 h (C), CNE2 cells were cultured with 50 U/ml IFNγ for the indicated incubation time (D). Cells were harvested and IDO expression was analyzed by western blotting using anti-IDO antibodies. Data are representative of three individual experiments.