Figure 2

PHA665752 inhibits SH-EP cell migration and proliferation/cell-survival. (A) Migration of SH-EP cells on vitronectin (10 ug/ml) was assessed in the transwell chemotaxis assay with increasing concentration gradients of HGF. (B) Migration of SH-EP vs. SY5Y was assessed on vitronectin (10 ug/ml) in the transwell chemotaxis assay in the presence of 200 ng/ml HGF either with or without pre-incubation with PHA665752. SY5Y cells were transiently transfected with empty pMOG vector or with either 0.5 ug or 1 ug of c-Met cDNA; inserts show photomicrographs (20× magnification) of migrated cells under specified conditions. (C) HGF-induced migration of SH-EP cells was measured in the transwell chemotaxis assay after either 60 minute pre-incubation or during continuous exposure(**) to PHA665752. (D) HGF-induced proliferation/cell-survival of SH-EP cells was measured by MTT assay either with or without increasing concentrations of PHA665752 over 72 hrs. Data represent mean +/- SD for triplicate independent experiments.