Figure 2

Immunoprecipitation studies. (A) Control IgG1, F-E2G5 and anti-GFP-tag antibodies were added to binding reactions with YB-1-GFP protein. Immunoblotting (IB) was carried out with anti-GFP antibody. (B) HEK293 cell protein extracts containing endogenous YB-1 protein were incubated with control IgG1, F-E2G5 and polyclonal anti-YB-1(C-term) antibodies and immunoprecipitated using pansorbin. Detection of protein pull-down was accomplished by immunoblotting with anti-YB-1(C-term) antibody. Sample buffers utilized were reducing (left) or non-reducing (right). Recombinant YB-1 protein was run aside w/o immunoprecipitation to visualize relative mobilities. "*" indicated mobilities of immunoglobulin heavy and light chains.