Protective effect induced by immunization with ECD/Her2 in HHD mice. A. Kaplan-Meier curve was plotted for tumor free analysis. Unbroken line represents ECD/Her2 vaccinated mice and broken line PBS vaccinated mice (control group). B. Tumor growth in groups of vaccinated mice. The volume of individual tumors was calculated as (length × width2)/2. Curves represent individual mice. Black square represents ECD/Her2 vaccinated mice and open circle PBS vaccinated mice (control group).