Vaccination with the mannosylated ECD/Her2 expressed in P. pastoris elicits a humoral response. Anti-ECD/Her2 antibodies in the sera of mice immunized with ECD/Her2 or PBS, as measured with enzyme-linked immunosorbent assay (ELISA). Sera were collected at various times. Pooled sera from the two groups of mice were assayed at a 1:1000 dilution. Columns and error bars represent the means and the range of values obtained from 3 independent experiments. ECD mice: ECD/Her2 vaccinated mice. PBS mice: PBS vaccinated mice (control group).