Re-expression of Prostasin and Protease-inactive Variant in KU-7 Cells Up-regulates E-cadherin Transcription. A. Western blots for prostasin and GAPDH for the KU-7 lentiviral sublines. Sample loading order was as indicated in the figure, 20 μg of total protein from each cell lysate were loaded in each lane. The nitrocellulose membrane was re-blotted separately for each target protein. B. qRT-PCR analysis of E-cadherin mRNA expression in the KU-7 lentiviral sublines. The data columns represent E-cadherin mRNA expression, shown as relative levels per GAPDH mRNA copy. The asterisks indicate a statistical difference (p < 0.05, n = 3) in the expression levels when compared with that of the KU-7/LacZ subline.